技术支持

技术支持

Immunogenicity and protective efficacy induced by self-amplifying mRNA vaccines encoding bacterial antigens

  • 分类:文献库
  • 作者:
  • 来源:ScienceDirect
  • 发布时间:2022-01-26 13:37
  • 访问量:

Immunogenicity and protective efficacy induced by self-amplifying mRNA vaccines encoding bacterial antigens

详情

Immunogenicity and protective efficacy induced by self-amplifying mRNA vaccines encoding bacterial antigens

Highlights

  • SAM vaccines were engineered to express prototype bacterial antigens from GAS and GBS.
  • Mice immunized with both vaccines produced fully functional serum antibodies.
  • Antibodies elicited by SAM vaccines conferred protection in mouse infection models.
  • SAM vaccines have the potential to be used for a broad range of targets.

Abstract

Nucleic acid vaccines represent an attractive approach to vaccination, combining the positive attributes of both viral vectors and live-attenuated vaccines, without the inherent limitations of each technology. We have developed a novel technology, the Self-Amplifying mRNA (SAM) platform, which is based on the synthesis of self-amplifying mRNA formulated and delivered as a vaccine. SAM vaccines have been shown to stimulate robust innate and adaptive immune responses in small animals and non-human primates against a variety of viral antigens, thus representing a safe and versatile tool against viral infections. To assess whether the SAM technology could be used for a broader range of targets, we investigated the immunogenicity and efficacy of SAM vaccines expressing antigens from Group A (GAS) and Group B (GBS) Streptococci, as models of bacterial pathogens. Two prototype bacterial antigens (the double-mutated GAS Streptolysin-O (SLOdm) and the GBS pilus 2a backbone protein (BP-2a)) were successfully expressed by SAM vectors. Mice immunized with both vaccines produced significant amounts of fully functional serum antibodies. The antibody responses generated by SAM vaccines were capable of conferring consistent protection in murine models of GAS and GBS infections. Inclusion of a eukaryotic secretion signal or boosting with the recombinant protein resulted in higher specific-antibody levels and protection. Our results support the concept of using SAM vaccines as potential solution for a wide range of both viral and bacterial pathogens, due to the versatility of the manufacturing processes and the broad spectrum of elicited protective immune response.

相关文件

  • Immunogenicity-and-protective-efficacy-induced-by-self-amplifying-_2017_Vacc.pdf

    大小:
  • Immunogenicity-and-protective-efficacy-of-a-single-dose-live-non-pat_2017_Va.pdf

    大小:
上一页
1

底部联系方式信息(左)

发布时间:2022-01-27 11:34:07

400-687-1881

公司电话:010-87875910

公司邮箱:info@bio-life.cn

公司官网:www.acciusa.cn   www.bio-life.cn

公司地址:北京市大兴区中关村科技园区大兴生物医药产业基地

华佗路50号院18幢

这是描述信息

关注官方微信

这是描述信息

关注手机网站